method of screening plant extract with diuretic potential
method of screening plant extract with diuretic potential
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method of screening plant extract with diuretic potential

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method of screening plant extract with diuretic potential ...

29 Oct 2012 , To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively , the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application , Total RNA was extracted with the PureLink Micro-to-Midi Total RNA Purification System with on-column.

International Journal of Pharmacological Screening Methods

The plant materials were air dried, powdered and methanol extract at the dose of 200mg/kg and 400mg/kg body weight administered to mice. Methanol crude extract showed remarkable diuretic

Phytochemical screening and in vitro bioactivity of ...

extract of the plant. The presence of phlobatannins sug-gests the diuretic property of the plant (Okuda, 1991). The foregoing would suggest the possible utilization of C. aconitifolius as diuretic agent. It was not really surprising that flavonoids were found absent for both forms of extracts. The sole greenish appearance of this leafy

Biological screening of various medicinal plant extracts ...

screening and testing plant extracts (4). Extracts from a broad spectrum of plant species contain substances that possess antitumor activity (1). New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities (5). Increasingly, natural products have potential for the treatment of cancer.

PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX MEDICINAL ...

Methods: Screening of six selected medicinal plants was performed for the presence of tannins, flavonoids, ... Preliminary phytochemical tests for plant extracts Phytoconstituents Test Observation Tannins ... reduces appetite and behaves as diuretic [23]. It

The screening analysis of antiradical activity of some ...

1. Postepy Hig Med Dosw (Online). 2006;60:563-70. The screening analysis of antiradical activity of some plant extracts. Sroka Z(1). Author information: (1)Department of Pharmacognosy, Wrocław Medical University, Wrocław, Poland. [email protected] INTRODUCTION: There is a need for screening studies in order to select plant extracts or plant raw materials with strong antiradical ...

SCREENING METHODS IN THE STUDY OF ANTIMICROBIAL

3 Screening Methods in the Study of Antimicrobial Properties of Medicinal Plants deposited into well cut into the agar and can be used with large number of extracts and/or large number of bacterial isolates (Dorman and Deans, 2000).

Screening of Plant Extracts for Antioxidant Activity: a ...

Three methods widely employed in the evaluation of antioxidant activity, namely 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS‐GC) and β‐carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts.

method of screening plant extract with diuretic potential

evaluation of the diuretic activity of the aqueous and 80 thus it is presumed to be advantageous to ‘pretreat’ or ‘prime’ the test animals with various fluids in screening agents for potential diuretic activity the aqueous and 80 methanol extracts of the leaves of ajuga remota showed an increase in urine volume that appeared to vary with dose and time as well as the nature of the extract

International Journal of Pharmacological Screening Methods

The plant materials were air dried, powdered and methanol extract at the dose of 200mg/kg and 400mg/kg body weight administered to mice. Methanol crude extract showed remarkable diuretic

Evaluation of antioxidant and diuretic activities of ...

In the present study, the diuretic and antioxidant potential of Clitoria ternatea leaf extracts were evaluated. Preliminary phytochemical screening of three solvent extracts [methanol (MCTL), chloroform, and aqueous) of the leaves revealed that the extracts were rich in alkaloids, glycosides, carbohydrates, flavonoids, and saponins. Among the various

Diuretic potential of various extracts of Oxystelma ...

The petroleum ether extract was found to possess the most effective diuretic activity, thereby supporting the traditional claim of the plant as a diuretic. Phytochemical screening of this extract...

The screening analysis of antiradical activity of some ...

MATERIAL/METHODS: The intensity of antiradical activity of extracts was investigated using DPPH* (1,1-diphenyl-2-picrylhydrazyl) radical as a substrate. The antiradical activity unit was defined and the number of antiradical activity units EAU(515) per 1 mg of plant extract and TAU(515) per 1 g of plant raw materials were calculated.

In-vitro qualitative and quantitative analysis of certain ...

Materials and Methods Chemical compounds having diuretic and antioxidant property of plants and or plant origin were screened for their in vitro diuretic and antioxidant potential. Screening was done on the basis of available scientific literature and indigenous technical knowledge of common people. Bio-organics of plant origin were

Evaluation of Diuretic Potential of Aqueous Root Extracts ...

Background: Tecoma stans a medicinal plant is used for its various pharmacological potential use to treat various ailments. Objective: To evaluate the diuretic potential of aqueous extracts of Tecomastans roots in normal rats after acute and sub-chronic oral administration. Materials and methods: Aqueous extracts of Tecomastans(200 and 400 mg/kg) along with the reference drug, furosemide (10 ...

PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX MEDICINAL ...

Terpenoids 2ml extract + 2ml (CH 3CO)2O + 2-3 drops conc. H2SO 4 Deep red coloration Saponins (Foam Test) (a) 5ml extract + 5ml H 2O + heat Froth appears (b) 5ml extract + Olive oil (few drops) Emulsion forms Steroids (Salkowski Test) 2ml extract + 2ml CHCl3 + 2ml H 2SO 4 (conc.) Reddish brown ring at the junction Phlobatannins (Precipitate Test)

Phytochemical Screening and Analysis of Selected

Froth test for saponins glycosides: By using distilled water the plant extract is diluted and this was shaken for 15 minutes in graduated cylinder. The presence of saponins was indicated by the formation of 1 cm layer of foam.

Avoiding pitfalls in determining antimicrobial activity

22-05-2019  Using resazurin as a measure of growth. The need for a different method to examine plant extracts was identified and discussed by two papers published in 1988. Mann and Markham [ 12] worked on essential oils and applied resazurin, a redox indicator that is used in the dairy industry.

method of screening plant extract with diuretic potential

evaluation of the diuretic activity of the aqueous and 80 thus it is presumed to be advantageous to ‘pretreat’ or ‘prime’ the test animals with various fluids in screening agents for potential diuretic activity the aqueous and 80 methanol extracts of the leaves of ajuga remota showed an increase in urine volume that appeared to vary with dose and time as well as the nature of the extract

The screening analysis of antiradical activity of some ...

MATERIAL/METHODS: The intensity of antiradical activity of extracts was investigated using DPPH* (1,1-diphenyl-2-picrylhydrazyl) radical as a substrate. The antiradical activity unit was defined and the number of antiradical activity units EAU(515) per 1 mg of plant extract and TAU(515) per 1 g of plant raw materials were calculated.

In-vitro qualitative and quantitative analysis of certain ...

Materials and Methods Chemical compounds having diuretic and antioxidant property of plants and or plant origin were screened for their in vitro diuretic and antioxidant potential. Screening was done on the basis of available scientific literature and indigenous technical knowledge of common people. Bio-organics of plant origin were

Evaluation of Diuretic Potential of Aqueous Root Extracts ...

Background: Tecoma stans a medicinal plant is used for its various pharmacological potential use to treat various ailments. Objective: To evaluate the diuretic potential of aqueous extracts of Tecomastans roots in normal rats after acute and sub-chronic oral administration. Materials and methods: Aqueous extracts of Tecomastans(200 and 400 mg/kg) along with the reference drug, furosemide (10 ...

Phytochemical screening by FTIR spectroscopic analysis of ...

Phytochemical screening by FTIR spectroscopic analysis of leaf extracts ... IR spectroscopy method. ... Dried powder of different solvent extracts of each plant materials were used for FTIR analysis. 10 mg of the dried extract powder was encapsulated in 100 mg of .

Preliminary phytochemical screening, Antibacterial ...

The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azadirachta indica. The antibacterial potential of the methanol leaf extract of the studie

Phytochemical Screening and Analysis of Selected

Froth test for saponins glycosides: By using distilled water the plant extract is diluted and this was shaken for 15 minutes in graduated cylinder. The presence of saponins was indicated by

Antimicrobial potentials of medicinal plant’s extract

01-11-2019  The MIC values of plant extracts were found to exhibit significant at 0.35–0.80 mg/mL. Among the tested plants, Albizia lebbeck, Cleistanthus collinus, Emblica officinalis, Eucalyptus deglupta, Eupatorium odoratum, Oxalis corniculata and Hevea brasiliensis were reported to show the minimum MIC values of 0.35–0.60 mg/mL.

Development of a method to screen and isolate potential ...

Development of a method to screen and isolate potential xanthine oxidase inhibitors from Panax japlcus var via ultrafiltration liquid chromatography combined with counter-current chromatography. Li S(1), Tang Y(2), Liu C(3), Li J(4), Guo L(5), Zhang Y(2).

Antibacterial Activity of Leaf Extracts of Baeckea ...

Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a